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includes a greatest catalytic exercise with only one divalent cation bound to the active site. These final results are consistent with the absence of the two histidines at the conclusion of β-strand a person along with the resolve of the x-ray construction on the Zn-NagA that displays a single Zn bound to the Mβ-website (21). The apo-enzyme was incubated with three equivalents of Fe2+, Mn2+, Ni2+, Cd2+, Co2+ or Zn2+ as well as the steel-substituted kinds of NagA have been located to consist of roughly a person equivalent of steel for each protein subunit just after elimination of the surplus steel by passage by way of a PD-ten column.
m profile (Desk 3). These results are fully in keeping with the conclusion that the second ionization noticed inside the pH-amount profile for log k
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